Gene transfer and genetic modification of embryonic stem cells by Cre‐ and Cre‐PR‐expressing MESV‐based retroviral vectors
Identifieur interne : 000838 ( Main/Exploration ); précédent : 000837; suivant : 000839Gene transfer and genetic modification of embryonic stem cells by Cre‐ and Cre‐PR‐expressing MESV‐based retroviral vectors
Auteurs : Stelios Psarras [France, Grèce] ; Niki Karagianni [France] ; Christoph Kellendonk [Allemagne] ; François Tronche [Allemagne] ; François Oic Cosset [France] ; Carol Stocking [Allemagne] ; Volker Schirrmacher [Allemagne] ; Harald Von Boehmer [France, États-Unis] ; Khashayarsha Khazaie [France, États-Unis]Source :
- The Journal of Gene Medicine [ 1099-498X ] ; 2004-01.
English descriptors
- KwdEn :
Abstract
Background: Genetic modification of embryonic stem (ES) cells represents a powerful tool for transgenic and developmental experiments. We report that retroviral constructs based on murine embryonal stem cell virus (MESV) can efficiently deliver and express Cre recombinase or a post‐translationally inducible Cre‐Progesterone receptor (Cre.PR) fusion in mouse fibroblasts and ES cells. Methods: To study the vectors a sensitive reporter cell line, 3TZ, was derived from the murine 3T6 fibroblast line that expresses β‐galactosidase only upon Cre‐mediated recombination. This was used together with the ROSA26‐R ES cell Cre‐reporter system or unmodified mouse ES cells as targets of infection. Efficiency of gene transfer was evaluated immunohistochemically by the use of an anti‐Cre polyclonal antibody, and by monitoring the expression of β‐galactosidase. Results: Infection of the 3TZ cells with high titer 718C or 719CP virus revealed efficient gene transduction of constitutive or hormone‐inducible recombinase activity, respectively. The vectors efficiently transduced murine ES cells with Cre, Cre‐PR (fusion of Cre and progesterone receptor) or β‐galactosidase. Cre‐mediated recombination in more than 60% of ROSA26‐R ES cells was achieved when infected by a VSV‐G‐pseudotyped MESV retrovirus at MOI of 50. Conclusions: The MESV‐based retroviral systems, when combined with hormone inducible Cre, represent efficient tools for the transfer of Cre activity in ES cells. Copyright © 2003 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jgm.442
Affiliations:
- Allemagne, France, Grèce, États-Unis
- Auvergne-Rhône-Alpes, Bade-Wurtemberg, District de Karlsruhe, Massachusetts, Rhône-Alpes, Île-de-France
- Boston, Heidelberg, Lyon, Paris
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Gene transfer and genetic modification of embryonic stem cells by Cre‐ and Cre‐PR‐expressing MESV‐based retroviral vectors</title><author><name sortKey="Psarras, Stelios" sort="Psarras, Stelios" uniqKey="Psarras S" first="Stelios" last="Psarras">Stelios Psarras</name></author><author><name sortKey="Karagianni, Niki" sort="Karagianni, Niki" uniqKey="Karagianni N" first="Niki" last="Karagianni">Niki Karagianni</name></author><author><name sortKey="Kellendonk, Christoph" sort="Kellendonk, Christoph" uniqKey="Kellendonk C" first="Christoph" last="Kellendonk">Christoph Kellendonk</name></author><author><name sortKey="Tronche, Francois" sort="Tronche, Francois" uniqKey="Tronche F" first="François" last="Tronche">François Tronche</name></author><author><name sortKey="Cosset, Francois Oic" sort="Cosset, Francois Oic" uniqKey="Cosset F" first="François Oic" last="Cosset">François Oic Cosset</name></author><author><name sortKey="Stocking, Carol" sort="Stocking, Carol" uniqKey="Stocking C" first="Carol" last="Stocking">Carol Stocking</name></author><author><name sortKey="Schirrmacher, Volker" sort="Schirrmacher, Volker" uniqKey="Schirrmacher V" first="Volker" last="Schirrmacher">Volker Schirrmacher</name></author><author><name sortKey="Boehmer, Harald Von" sort="Boehmer, Harald Von" uniqKey="Boehmer H" first="Harald Von" last="Boehmer">Harald Von Boehmer</name></author><author><name sortKey="Khazaie, Khashayarsha" sort="Khazaie, Khashayarsha" uniqKey="Khazaie K" first="Khashayarsha" last="Khazaie">Khashayarsha Khazaie</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:DDFA1B3954E5822528029DF079EA805025138BD8</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1002/jgm.442</idno><idno type="url">https://api.istex.fr/document/DDFA1B3954E5822528029DF079EA805025138BD8/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000529</idno><idno type="wicri:Area/Main/Curation">000526</idno><idno type="wicri:Area/Main/Exploration">000838</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000838</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Gene transfer and genetic modification of embryonic stem cells by Cre‐ and Cre‐PR‐expressing MESV‐based retroviral vectors</title><author><name sortKey="Psarras, Stelios" sort="Psarras, Stelios" uniqKey="Psarras S" first="Stelios" last="Psarras">Stelios Psarras</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U373, Institut Necker, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Grèce</country><wicri:regionArea>Current Address: Allergy Unit, 2nd Pediatric Clinic, University of Athens</wicri:regionArea><wicri:noRegion>University of Athens</wicri:noRegion></affiliation></author><author><name sortKey="Karagianni, Niki" sort="Karagianni, Niki" uniqKey="Karagianni N" first="Niki" last="Karagianni">Niki Karagianni</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U373, Institut Necker, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Kellendonk, Christoph" sort="Kellendonk, Christoph" uniqKey="Kellendonk C" first="Christoph" last="Kellendonk">Christoph Kellendonk</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Tronche, Francois" sort="Tronche, Francois" uniqKey="Tronche F" first="François" last="Tronche">François Tronche</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Cosset, Francois Oic" sort="Cosset, Francois Oic" uniqKey="Cosset F" first="François Oic" last="Cosset">François Oic Cosset</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Retroviral Vectorology and Gene Therapy, INSERM U412, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Stocking, Carol" sort="Stocking, Carol" uniqKey="Stocking C" first="Carol" last="Stocking">Carol Stocking</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division for Cellular and Viral Genetics, Heinrich Pette Institute for Experimental Virology and Immunology, University of Hamburg</wicri:regionArea><wicri:noRegion>University of Hamburg</wicri:noRegion><wicri:noRegion>University of Hamburg</wicri:noRegion></affiliation></author><author><name sortKey="Schirrmacher, Volker" sort="Schirrmacher, Volker" uniqKey="Schirrmacher V" first="Volker" last="Schirrmacher">Volker Schirrmacher</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division of Cellular Immunology, Tumor Immunology Program, German Cancer Research Center, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Boehmer, Harald Von" sort="Boehmer, Harald Von" uniqKey="Boehmer H" first="Harald Von" last="Boehmer">Harald Von Boehmer</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U373, Institut Necker, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cancer Immunology and Aids, Dana Farber Cancer Institute, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Khazaie, Khashayarsha" sort="Khazaie, Khashayarsha" uniqKey="Khazaie K" first="Khashayarsha" last="Khazaie">Khashayarsha Khazaie</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U373, Institut Necker, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cancer Immunology and Aids, Dana Farber Cancer Institute, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">The Journal of Gene Medicine</title><title level="j" type="sub">A cross‐disciplinary journal for research on the science of gene transfer and its clinical applications</title><title level="j" type="abbrev">J. Gene Med.</title><idno type="ISSN">1099-498X</idno><idno type="eISSN">1521-2254</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2004-01">2004-01</date><biblScope unit="volume">6</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="32">32</biblScope><biblScope unit="page" to="42">42</biblScope></imprint><idno type="ISSN">1099-498X</idno></series><idno type="istex">DDFA1B3954E5822528029DF079EA805025138BD8</idno><idno type="DOI">10.1002/jgm.442</idno><idno type="ArticleID">JGM442</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-498X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cre recombinase</term><term>Cre.PR fusion</term><term>ES cells</term><term>MESV retroviral vectors</term><term>adenoviral vectors</term><term>gene transfer</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Genetic modification of embryonic stem (ES) cells represents a powerful tool for transgenic and developmental experiments. We report that retroviral constructs based on murine embryonal stem cell virus (MESV) can efficiently deliver and express Cre recombinase or a post‐translationally inducible Cre‐Progesterone receptor (Cre.PR) fusion in mouse fibroblasts and ES cells. Methods: To study the vectors a sensitive reporter cell line, 3TZ, was derived from the murine 3T6 fibroblast line that expresses β‐galactosidase only upon Cre‐mediated recombination. This was used together with the ROSA26‐R ES cell Cre‐reporter system or unmodified mouse ES cells as targets of infection. Efficiency of gene transfer was evaluated immunohistochemically by the use of an anti‐Cre polyclonal antibody, and by monitoring the expression of β‐galactosidase. Results: Infection of the 3TZ cells with high titer 718C or 719CP virus revealed efficient gene transduction of constitutive or hormone‐inducible recombinase activity, respectively. The vectors efficiently transduced murine ES cells with Cre, Cre‐PR (fusion of Cre and progesterone receptor) or β‐galactosidase. Cre‐mediated recombination in more than 60% of ROSA26‐R ES cells was achieved when infected by a VSV‐G‐pseudotyped MESV retrovirus at MOI of 50. Conclusions: The MESV‐based retroviral systems, when combined with hormone inducible Cre, represent efficient tools for the transfer of Cre activity in ES cells. Copyright © 2003 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>France</li><li>Grèce</li><li>États-Unis</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li><li>Massachusetts</li><li>Rhône-Alpes</li><li>Île-de-France</li></region><settlement><li>Boston</li><li>Heidelberg</li><li>Lyon</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Psarras, Stelios" sort="Psarras, Stelios" uniqKey="Psarras S" first="Stelios" last="Psarras">Stelios Psarras</name></region><name sortKey="Boehmer, Harald Von" sort="Boehmer, Harald Von" uniqKey="Boehmer H" first="Harald Von" last="Boehmer">Harald Von Boehmer</name><name sortKey="Cosset, Francois Oic" sort="Cosset, Francois Oic" uniqKey="Cosset F" first="François Oic" last="Cosset">François Oic Cosset</name><name sortKey="Karagianni, Niki" sort="Karagianni, Niki" uniqKey="Karagianni N" first="Niki" last="Karagianni">Niki Karagianni</name><name sortKey="Khazaie, Khashayarsha" sort="Khazaie, Khashayarsha" uniqKey="Khazaie K" first="Khashayarsha" last="Khazaie">Khashayarsha Khazaie</name></country><country name="Grèce"><noRegion><name sortKey="Psarras, Stelios" sort="Psarras, Stelios" uniqKey="Psarras S" first="Stelios" last="Psarras">Stelios Psarras</name></noRegion></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Kellendonk, Christoph" sort="Kellendonk, Christoph" uniqKey="Kellendonk C" first="Christoph" last="Kellendonk">Christoph Kellendonk</name></region><name sortKey="Schirrmacher, Volker" sort="Schirrmacher, Volker" uniqKey="Schirrmacher V" first="Volker" last="Schirrmacher">Volker Schirrmacher</name><name sortKey="Stocking, Carol" sort="Stocking, Carol" uniqKey="Stocking C" first="Carol" last="Stocking">Carol Stocking</name><name sortKey="Tronche, Francois" sort="Tronche, Francois" uniqKey="Tronche F" first="François" last="Tronche">François Tronche</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Boehmer, Harald Von" sort="Boehmer, Harald Von" uniqKey="Boehmer H" first="Harald Von" last="Boehmer">Harald Von Boehmer</name></region><name sortKey="Khazaie, Khashayarsha" sort="Khazaie, Khashayarsha" uniqKey="Khazaie K" first="Khashayarsha" last="Khazaie">Khashayarsha Khazaie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/SchutzV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000838 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000838 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= SchutzV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:DDFA1B3954E5822528029DF079EA805025138BD8
|texte= Gene transfer and genetic modification of embryonic stem cells by Cre‐ and Cre‐PR‐expressing MESV‐based retroviral vectors
}}
| This area was generated with Dilib version V0.6.38. |  |